Analysis of ELA-DRB Exon 2 Polymorphism by PCR-RFLP.
نویسندگان
چکیده
منابع مشابه
Frequency of Human Platelet Antigens (HPA-2/3/5) Polymorphism in Iranians Evaluated by RFLP-PCR
ackground: Knowing the human platelet antigens (HPA) and genes frequency in different populations is important not only for population studies but also for clinical transfusion practice. HPA genes frequency in Iran is not evaluated, so we investigated the HPA-2, HPA-3, and HPA-5 frequency and their polymorphism. Materials and Methods: DNA from 120 Iranians (99 Iranian blood donors and 21 patie...
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this study was conducted to determine polymorphism of gola-drb3 in nadushan goat using pcr-rflp. blood samples were randomly taken from 100 goats. dna was extracted from the blood samples using diatom dna prep kit. spectrophotometer and 1% agarose gel were used for determining its quantities and qualities. exon 2 of drb 3.2 gene encompassing 285 bp was amplified with heminested-pcr method in tw...
متن کاملIntraspecies polymorphism of Cryptosporidium parvum revealed by PCR-restriction fragment length polymorphism (RFLP) and RFLP-single-strand conformational polymorphism analyses.
A glycoprotein (Cpgp40/15)-encoding gene of Cryptosporidium parvum was analyzed to reveal intraspecies polymorphism within C. parvum isolates. Forty-one isolates were collected from different geographical origins (Japan, Italy, and Nepal) and hosts (humans, calves, and a goat). These isolates were characterized by means of DNA sequencing, PCR-restriction fragment length polymorphism (PCR-RFLP),...
متن کاملFrequency of Human Platelet Antigens (HPA-2/3/5) Polymorphism in Iranians Evaluated by RFLP-PCR
Background: Knowing the human platelet angens (HPA) and genes frequency in different populaons is important not only for populaon studies but also for clinical transfusion pracce. HPA genes frequency in Iran is not evaluated, so we invesgated the HPA-2, HPA-3, and HPA-5 frequency and their polymorphism. Materials and Methods: DNA from 120 Iranians (99 Iranian blood donors and 21 paents wi...
متن کاملPolymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep
The aim of present study was to investigate myostatin gene polymorphism and its association with yearling weight records in Zel sheep using PCR-RFLP and PCR-SSCP methods. Blood samples were collected from 200 Zel sheep, randomly, and DNA was extracted using modified salting out method. Polymerase chain reaction was carried out to amplify 337, 222, and 311 bp fragments, respectively, comprising ...
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ژورنال
عنوان ژورنال: Journal of Equine Science
سال: 1999
ISSN: 1340-3516,1347-7501
DOI: 10.1294/jes.10.13